Inhibition of nucleoside diphosphate kinase in rat liver mitochondria by added 3′-azido-3′-deoxythymidine

Abstract

The effect of 3′-azido-3′-deoxythymidine on nucleoside diphosphate kinase of isolated rat liver mitochondria has been studied. This is done by monitoring the increase in the rate of oxygen uptake by nucleoside diphosphate (TDP, UDP, CDP or GDP) addition to mitochondria in state 4. It is shown that 3′-azido-3′-deoxythymidine inhibits the mitochondrial nucleoside diphosphate kinase in a competitive manner, with a K i value of about 10 μM as measured for each tested nucleoside diphosphate. It is also shown that high concentrations of GDP prevent 3′-azido-3′-deoxythymidine inhibition of the nucleoside diphosphate kinase.