Abstract
The possibility of protecting cook-chill foods with microbial cultures against the risk of botulism was demonstrated. Three commercial soups were incubated with Clostridium botulinum 17B (103 spores/g) and protective cultures (PCs) during 10–15 days at 10°C. The PCs populations were enumerated on M17, MRS and maltose tryptic soy agar, C. botulinum—on sorbitol tryptic soy agar, botulinal toxin was detected by the immunoassay, bacteriocins—by well diffusion assay. C. botulinum did not grow in two soups with low pH (5.2–5.5) and was unaffected by the PCs. In seafood chowder (pH 6.2) C. botulinum populations reached 108 cfu/g. The co-incubation with the PCs, nisin-producing Lactococcus lactis (107 cfu/g) or pediocin-producing Pediococcus pentosaceus (3×108 cfu/g) singularly and as a mixture, prevented toxigenesis as well as reduced the product pH to 4.8–5.0 and C. botulinum populations to undetectable levels. Color, mouth-feel, texture, flavor and the overall acceptability of seafood chowder was not affected by the presence of the PCs.
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