Inhibition of nitrification in forest soil by monoterpenes

Abstract

Nitrate production was detected in untreated soil of a Norway spruce (Picea abies L.) stand only after clear-cutting the stand. The aim of this study was to determine whether allelochemical inhibition of nitrification by monoterpenes played any role in inhibiting nitrification in the stand. Therefore, soils from a clear-cut plot and from a forest plot were studied. In the field, monoterpenes (mostly α- and β-pinenes), measured by soil microair diffusive samplers, were intensively produced in the forest plot, but not in the clear-cut plot. In the laboratory, soil samples taken from the forest plot produced only small amounts of monoterpenes, indicating that monoterpenes were mainly produced by the roots and not to great extent by the soil microbial population. The effect of a mixture of monoterpenes (seven major monoterpenes detected in the field) on net nitrification, net N mineralization and denitrification activities of soil from the clear cut plot, and on carbon mineralization of soils from both the forest and clear-cut plots, was studied in the laboratory. In both aerobic incubation experiments and in soil suspensions with excess NH4-N, nitrification was inhibited by exposure to the vapours of monoterpenes at similar concentrations at which they had been detected in forest plot. This indicates direct inhibition of nitrification by monoterpenes. Exposure to monoterpenes did not affect denitrification. However, it increased respiration activity of both soils. This could also indicate indirect inhibition of nitrification by monoterpenes, due to immobilization of mineral N. Thus it seems that monoterpenes could play a role in inhibiting nitrification in the forest soil.