Abstract

The activity of soluble and membrane-bound ATPase obtained from corn roots was in vivo markedly inhibited by N,N' -dicyclohexylcanbodiimide (DCCD) and W0<sub>4</sub><sup>2-</sup> ions. DCCD (2.5 X 10<sup>-5</sup> M) added to the nutrient solution strongly decreased in vivo nitrate reductase (NR) activity after 12-h growth of plants while it had no effect in experiments <em>in vitro</em> on NR activity. Tungsten in a concentration of 10<sup>-4</sup> M completely blocked NR activity after 24 h. In the above used concentrations neither DCCD nor W0<sub>4</sub><sup>2-</sup> inhibited completely N0<sub>3</sub><sup>-</sup> absorption by corn roots. The results suggest that there must exist in corn roots another or an additional mechanism of N0<sub>3</sub><sup>-</sup> assimilation apart from of that proposed by Butz and Jackson (1977).

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