Abstract
A single intraperitoneal injection of 30, 60, or 90 mg/kg of sodium pentobarbital into male rats produced an appreciable inhibition of monoamine oxidase activity of liver and heart. The brain and kidney activities were practically unaffected. Chronic administration of pentobarbital, 30 mg/kg daily for 20 days, did, however, produce an inhibition of brain monoamine oxidase (MAO) which was maximal 48 h after the last injection. In all other experiments with a single dose of barbiturate the maximal inhibition of MAO appeared 15–30 min after treatment.Sodium pentobarbital inhibited MAO activity in vitro when added to a liver mitochondrial preparation. High concentrations of barbiturate had to be used for the in vitro experiments. The Ki value for sodium pentobarbital was 1.21 × 10−3 M while the Km was 1.6 × 10−5 M. The observed inhibition was of a competitive nature. The inhibition is comparable with that observed for some flavoenzymes following addition of barbiturates.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
