Inhibition of mitogen induced blastogenesis by 5′-deoxy-5′-S-iso-butyl adenosine

Abstract

The mitogen induced blastogenesis of human and rabbit lymphocytes is strongly inhibited by 100 and 300 μM 5′-deoxy-5′-S-isobutyl-adenosine (SIBA) (I), a synthetic analogue of S-adenosyl-homocysteine (SAH) which had previously been shown to prevent oncogenic transformation of chicken fibroblasts by Rous Sarcoma virus (15). Addition of 300 μM SIBA even 1 to 2 days after stimulation by Con A or a Water soluble mitogen from Nocardia (NWSM), inhibited strongly the incorporation of [ 3H] thymidine. Lymphocytes recovered their ability to be stimulated by T or B mitogens after elimination of the inhibitor from the cultures.