Abstract
This chapter describes inhibition of mitochondrial F 1 -ATPase by Adenylyl Imidodiphosphate (AMPPNP).(AMPPNP is a close structural analog of adenosine triphosphate (ATP), the difference being that the bridge oxygen atom between the β and γ phosphorus atoms of ATP is replaced by an NH group in AMPPNP. The analog binds to ATP binding sites on mitochondrial F 1 - adenosine triphosphatase (ATPase) but F 1 does not catalyze its hydrolysis. AMPPNP can be described as an apparent one-way inhibitor of the mitochondrial ATPase. One-way inhibition of F 1 is also affected by the mitochondrial ATPase inhibitor protein, removal of tightly bound nucleotides, covalent modification by NBD-Cl, and with bacterial F 1 covalent modification by a 2', 3'-dialdehyde derivative of ATP. This chapter discusses several possible mechanisms for one-way inhibition and presents some results pertinent to the mechanism of inhibition of F 1 by AMPPNP.
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