Abstract
AbstractThe inhibitory effect of α‐tocopherol, β‐carotene, 2″‐O‐glycosyl isovitexin (2″‐O‐GIV), and butylated hydroxytoluene (BHT) on malonaldehyde (MA) formation from ω3 polyunsaturated fatty acids (PUFA) was determined by gas chromatography. The levels of MA formed from 1 mg each of octadecatetraenoic acid (ODTA), eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA) upon oxidation with Fenton's reagent were 29.8±1.5, 17.2±1.5, and 22.0±0.7 nmol, respectively. BHT was most effective toward protecting all three ω3 PUFA, whereas β‐carotene did not exhibit any inhibitory effect. 2″‐O‐GIV inhibited MA formation from EPA and DHA by 56 and 43%, respectively, showing the second greatest inhibitory activity after BHT. α‐Tocopherol inhibited MA formation from ODTA and DHA by 67 and 28%, respectively, but it did not show any activity toward EPA oxidation. The naturally occurring antioxidant, 2″‐O‐GIV, may be useful to prevent oxidation of ω3 PUFA.
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