Abstract
Cyclosporin (CsA) 4 4 ABBREVIATIONS CsA, cyclosporin-A; PEM, peritoneal exudate macrophage; MAF, macrophage activation factor; LPS, lipopolysaccharide; DMEM, Dulbecco's Modified Eagle's Medium; PBS, calcium, magnesium-free phosphate buffered saline; HBSS, calcium, magnesium-free Hank's Balanced Salt Solution; DMSO, dimethylsulfoxide; FCS, heat-inactivated fetal calf serum; IUDR, 5-iododeoxyuridine. , a fungal peptide used clinically for its immunosuppressive properties, was investigated for its ability to antagonize the activation of macrophages (PEM) to the tumoricidal state. The acquisition of tumoricidal properties by PEM challenged with macrophage activating factor (MAF) plus lipopolysaccharide (LPS) was inhibited in a dose-dependent fashion by CsA. Similarly, CsA antagonized activation of PEM exposed to the calcium ionophore, A23187. CsA also inhibited macrophage-mediated tumor cell cytolysis in a dose-dependent manner. These data indicate that in vitro , CsA can modulate directly the acquisition and expression of tumoricidal properties by PEM and suggests that the macrophage may be an important target cell for CsA in vivo .
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