Abstract

BackgroundThe consumer demands for better quality and safety of food products have given rise to the development and implementation of edible films. The use of antimicrobial films can be a promising tool for controlling L. monocytogenes on ready to eat products. The aim of this study was to develop effective antimicrobial films incorporating bioactive compounds from green and black teas into chitosan, for controlling L. monocytogenes ATCC 19115 on vacuum-packaged ham steak. The effectiveness of these antimicrobial films was evaluated at room temperature (20°C) for 10 days and at refrigerated temperature (4°C) for 8 weeks.ResultsThe HPLC results clearly show that relative concentrations of catechins and caffeine in green tea ranked EGCG>EGC>CAF>ECG>EC>C while in black tea extracts ranked CAF>EGCG>ECG>EGC>EC>C. The chitosan-coated plastic films incorporating green tea and black tea extracts shows specific markers identified by FTIR. Incorporating natural extracts into chitosan showed that the growth of L monocytogenes ATCC 19115 was inhibited. The efficacy of antimicrobial effect of tea extracts incorporated into chitosan-coated plastic film was dose dependent. However, chitosan-coated films without addition of tea extracts did not inhibit the growth of L. monocytogenes ATCC 19115. Chitosan-coated plastic films incorporating 4% Green tea extract was the most effective antimicrobial, reducing the initial counts from 3.2 to 2.65 log CFU/cm2 during room temperature storage and from 3.2 to 1–1.5 log CFU/cm2 during refrigerated storage.ConclusionsIncorporation of tea extracts into the chitosan-coated films considerably enhanced their effectiveness against L. monocytogenes ATCC 19115. 4% Green tea incorporated into chitosan-coated plastic film had a better antilisterial effect than 2% green tea or 2% and 4% black tea. Data from this study would provide new formulation options for developing antimicrobial packaging films using tea extracts to improve the microbiological safety and quality of ham steak during room and refrigerated storage.

Highlights

  • The consumer demands for better quality and safety of food products have given rise to the development and implementation of edible films

  • The treatment with 4% Chitosan-coated plastic film incorporating Green Tea extract (CHGT) was the most effective and completely inhibited the growth of L. monocytogenes ATCC 19115 after 8 weeks storage of 4°C, decreasing from 3.2 to 1 log CFU/cm2. These results demonstrate that chitosan-coated plastic films incorporated with 4% CHGT could be use to control L. monocytogenes ATCC 19115 on ham steaks

  • The results of this study clearly show that relative concentrations of catechins and caffeine in green tea ranked Epigallocatechin Gallate (EGCG) > EGC > CAF > Epicatechin Gallate (ECG) > EC > C while in black tea extracts ranked CAF > EGCG > ECG > EGC > EC > C

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Summary

Introduction

The consumer demands for better quality and safety of food products have given rise to the development and implementation of edible films. The aim of this study was to develop effective antimicrobial films incorporating bioactive compounds from green and black teas into chitosan, for controlling L. monocytogenes ATCC 19115 on vacuum-packaged ham steak. The effectiveness of these antimicrobial films was evaluated at room temperature (20°C) for 10 days and at refrigerated temperature (4°C) for 8 weeks. Chitosan is a natural polymer, which is non-toxic, biodegradable and biocompatible and has intrinsic antimicrobial activity; chitosan film alone had no inhibitory effect on the growth of L. monocytogenes when applied to the surface of ham steaks [1]. A new alternative, by incorporating of natural bioactive compounds into edible films is needed

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