Inhibition of lipogenesis in isolated hepatocytes by 3-amino-1,2,4-triazole

Abstract

3-Amino-1,2,4-triazole has been found to be an inhibitor of fatty acid synthesis by isolated rat hepatocytes. Half-maximal inhibition of fatty acid synthesis occurs at approximately 20 mM. Acetyl-CoA carboxylase activity in homogenates of hepatocytes is not affected by previous exposure of the intact cells to 3-amino-1,2,4-triazole. The drug opposes the activation of partially purified acetyl-CoA carboxylase by citrate, but does not influence enzyme activity in the absence of citrate. As compared to fatty acid synthesis, cholesterol synthesis by the hepatocytes is more drastically depressed by incubation of the cells with 3-amino-1,2,4-triazole. Half-maximal inhibition of cholesterogenesis occurs at approximately 5 mM 3-amino-1,2,4-triazole.