Abstract
Patch-clamp recording techniques were used to examine the effect of troglitazone on K ATP channel activity in Cambridge rat insulinoma-G1 (CRI-G1) insulin-secreting cells. In both inside-out and outside-out patch recordings, bath application of troglitazone reduced K ATP channel activity. This inhibition was independent of the membrane voltage and was poorly reversible. In whole-cell studies, troglitazone inhibited K ATP channel currents with an IC 50 of 697 ± 92 nM and an associated Hill coefficient of 1.2 ± 0.2. In current clamp recordings 10 μM troglitazone depolarised the CRI-G1 cell membrane by 36.8 ± 3.9 mV with a concomitant decrease in membrane conductance. However, in contrast to the rapid depolarisation produced by tolbutamide, the effects of troglitazone developed more slowly, usually taking 15–20 min to develop.
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