Inhibition of human breast and colorectal cancer cells by Viburnum foetens L. extracts in vitro

Abstract

ObjectiveTo investigate efficacy of Viburnum foetens (V. foetens) extracts against different cancer lines. MethodsThe crude extract and fractions of V. foetens are evaluated against MDA MB-468 and Caco-2 cancer cell lines by using MTT (3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide) assays. These extracts are also tested against breast carcinoma and human colon adenocarcinoma through NRU (neutral red uptake) assay. ResultsThe crude extract inhibited the cancerous cell growth in a dose dependent manner. From the MTT assay it is obvious that the ethylacetate fraction significantly inhibited the growth of Caco-2 (93.44%) cell. Similarly, the methanol and ethylacetate fractions shows 99% and 96% inhibition of MCF-7 and Caco-2 cell lines by NRU assay. Furthermore, the ethylacetate fraction also exhibited momentous inhibition of MDA MB-468 cells in both assays. Other fractions i.e. chloroform, hexane also inhibited cancer cell proliferation at a significant level. Natural products exhibited significant activity against multiple cancerous cells. ConclusionsIn this framework, we can speculate that the present study will be helpful in the identification and isolation of novel anticancer drug compounds from the crude extract (i.e., methanol and ethyl acetate fractions) of V. foetens.