Inhibition of Human Alveolar Macrophage Production of Leukotriene B4 by Acute In Vitro and In Vivo Exposure to Tobacco Smoke

Abstract

The number of neutrophils in the lungs of cigarette smokers is increased. This could be a consequence of the chemotactic mediator synthesis by alveolar macrophages (AM). In order to evaluate the possible role of leukotriene B4 (LTB4) in this condition, we studied the formation of LTB4 by nonsmokers' AM exposed in vitro and in vivo to cigarette smoke and by smokers' AM. In the absence of stimulus or upon stimulation, nonsmokers' AM exposed in vitro to tobacco smoke formed less LTB4 than did nonexposed AM, e.g., the cells incubated with arachidonic acid and ionophore produced, respectively, 241 +/- 132 and 425 +/- 106 pmol LTB4/10(6) cells (mean +/- SEM) (P less than 0.01). In other experiments, smokers' AM were incubated in absence of stimulus and produced less LTB4 than did nonsmokers' AM; during a 3-h incubation, smokers' and nonsmokers' adherent AM released, respectively, 3 +/- 2 and 40 +/- 28 pmol LTB4/10(6) cells (P less than 0.05). Similarly stimulated smokers' AM produced less LTB4 than did nonsmokers' AM, e.g., the cells incubated with arachidonic acid and ionophore formed, respectively, 225 +/- 41 and 573 +/- 150 pmol LTB4/10(6) cells (P less than 0.05). In a group including mild smokers and nonsmokers, in vivo exposure to the smoke of 4 cigarettes produced a decrease in the release of LTB4 by AM, e.g., in the presence of arachidonic acid and ionophore, nonexposed and exposed AM produced, respectively, 198 +/- 38 and 143 +/- 38 pmol LTB4/10(6) cells (P less than 0.05).(ABSTRACT TRUNCATED AT 250 WORDS)