Inhibition of granulocyte colony stimulating factor (G-CSF) adsorption to polyvinyl chloride using a nonionic surfactant

Abstract

The effect of poloxamer 407 (Pluronic® F-127), a nonionic surfactant, on the adsorption of granulocyte colony stimulating factor (G-CSF) to polyvinyl chloride (PVC) was assessed. G-CSF, at concentrations less than 15 μ/ml, was combined with human serum albumin (HSA), poloxamer 407, and HSA plus poloxamer 407 in dextrose 5% in water (D5W) and the percent of G-CSF bound to PVC compared with G-CSF in D5W alone. The concentrations of poloxamer 407 evaluated for their effect on G-CSF adsorption to PVC were 0.005, 0.05, and 0.5% (w/w), whereas solutions which contained HSA with or without poloxamer 407 employed HSA at a concentration of 2 mg/ml only. A concentration of 0.05% w/w poloxamer 407 in a solution of G-CSF in D5W provided a significant ( p < 0.05) decrease in the percent of G-CSF bound to PVC compared to a solution of G-CSF alone. Poloxamer 407 alone at a concentration of 0.05% w/w was just as effective as HSA alone (2 mg/ml) at inhibiting adsorption of G-CSF to PVC. However, inhibition of G-CSF surface adsorption to PVC was not dependent on the concentration of poloxamer 407 when the surfactant was combined with HSA. Incorporation of poloxamer 407 at each of the three concentrations selected in a G-CSF/HSA solution provided no additional reduction in G-CSF adsorption to PVC when compared to a G-CSF solution which contained only HSA at a concentration of 2 mg/ml. Over the G-CSF concentration range of 0.3–300 ng/ml, the amount of G-CSF adsorbed per square centimeter of PVC was significantly greater at 45°C compared to G-CSF solutions evaluated at 22°C. Poloxamer 407 at a concentration of 0.05% w/w may show promise as a solvent additive with which to minimize G-CSF adsorption to PVC.