Inhibition of Gastric Secretion by Extracts Containing Gastrin

Abstract

Summary A method is described for preparation of extracts containing gastrin from mucosa of the pyloric gland area of the stomach of hogs. These extracts stimulated secretion of acid from Heidenhain pouches in dogs when given by continuous intravenous injection in the dose range 2.5 to 40 gm. per hour (dose of gastrin expressed as wet weight of mucosa from which extract was derived). When given as single rapid intravenous injections to dogs secreting in response to continuous injection of gastrin extract or of histamine, these same extracts caused marked inhibition of secretion of acid. Inhibition of acid secretion was not associated with retching, vomiting, rise in rectal temperature, or fall in blood pressure. Doses as low as 6 gm. given as a rapid intravenous injection produced inhibition. Inhibition began within 15 minutes, reached a maximum of 80 to 90 per cent in 30 to 45 minutes and subsided in 2 to 3 hours. Extracts prepared by the same method from mucosa of the fundic gland area, duodenum, ileum, and colon, or from pancreas produced little or no inhibition of acid secretion. It cannot yet be determined whether the stimulatory and inhibitory actions of these extracts are caused by the same or different constituents, but reasons are given for favoring the view that they are the same.