Abstract
Abstract Comparisons were made of the ratios of the specific activities of stearic acid to oleic acid in adipose tissue and liver triglycerides of rats given methyl stearate-1-14C dissolved in 0.25 ml of corn oil and Sterculia foetida oil. The ratios were about 3 in the corn oil control animals compared to 31 to 43 in adipose tissue and 44 to 246 in liver triglycerides of the S. foetida test animals. When the labeled stearate was administered in 0.5 ml of S. foetida oil almost no label appeared in tissue oleic acid. The inhibition was also shown to be affected by sterculic acid in vitro with rat liver preparations. Evidence was obtained that the mechanism of inhibition is the irreversible binding of enzyme sulfhydryl groups by the cyclopropene group.
Highlights
Mercaptopropanoic acid with cyclopropene compounds and Comparisonswere made of the ratios of the specific activities of stearic acid to oleic acid in adipose tissue and liver triglycerides of rats given methyl stearate-lJ4C dissolved in 0.25 ml of corn oil and Sterculia foetida oil
A thiol compound,such as2-mercaptoethanol,can restorefull activity when addedto the inhibited system. These findings support the hypothesis that the increasein saturatedfatty acidsat the expenseof the correspondingmonoenesobservedwhen animalsingestsmallamountsof CFA is due to binding of the thiol groupsof acyl desaturaseby the cyclopropeneproups
A variety of biological effects has been observed in several speciesof animalsupon ingestion of even small quantities of cyclopropenefatty acids
Summary
Mercaptopropanoic acid with cyclopropene compounds and Comparisonswere made of the ratios of the specific activities of stearic acid to oleic acid in adipose tissue and liver triglycerides of rats given methyl stearate-lJ4C dissolved in 0.25 ml of corn oil and Sterculia foetida oil. A thiol compound,such as2-mercaptoethanol,can restorefull activity when addedto the inhibited system These findings support the hypothesis that the increasein saturatedfatty acidsat the expenseof the correspondingmonoenesobservedwhen animalsingestsmallamountsof CFA is due to binding of the thiol groupsof acyl desaturaseby the cyclopropeneproups. A pronouncedeffect of dietary CFAl on animal metabolismis an increasein the saturated fatty acids and a decreasein the correspondingmonoenesof tissuelipids. Even though this effect hasoften beennoted, very little hasbeendone to elucidate the mechanism. Parekh, and Meinke [2] have shownthat aerobicyeasts grown in a mediumcontaining liver extracts In both caseswe were able to demonstrate inhibition of the activity of fatty acyl desaturaseby CFA. Oil from seedsof Xterculia foetidu (CFA content about 60%) produceda mixture of fatty acidswith a compositionsimilarto
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