Inhibition of ethylene production in sunflower cell suspensions by the plant growth retardant BAS 111..W: Possible relations to changes in polyamine and cytokinin contents

Abstract

At a concentration of 10−5 mol · L−1 the triazole-type growth retardant BAS 111..W completely inhibited the transiently elevated ethylene production in the exponential growth phase of heterotrophic sunflower cell suspensions. This effect, which could not be restored by adding gibberellin A3, was accompanied by transiently increased levels of 1-aminocyclopropanecarboxylic acid (ACC) in the cells, which was increasingly converted to N-malonyl-ACC. Thus, the reactions from ACC to ethylene catalyzed by the ethylene-forming enzyme appeared to be blocked by the retardant. Concomitantly, higher endogenous levels of free spermidine and particularly spermine were found over control, whereas free putrescine, the direct precursor of both polyamines, simultaneously decreased. We assume that the remaining S-adenosylmethionine from ethylene biosynthesis was increasingly incorporated into spermidine and finally spermine. A further relation appears to exist between the reduced ethylene production and enhanced levels of cytokinins in the cells. The application of both BAS 111..W and aminoethoxyvinylglycine depressed ethylene formation while immunoreactive cytokinins from isopentenyladenosine-, trans-zeatin ribo-side-, and dihydrozeatin riboside-type increased. By additional treatment with ACC, the effects could partially be reversed. On the other hand, stimulation of ethylene production by ACC alone or ethephon considerably lowered cytokinin levels.