Inhibition of Ethylene Production by 2,4-Dinitrophenol and High Temperature

Abstract

2,4-Dinitrophenol (DNP) and high temperature (35 to 40 C) are known to inhibit C(2)H(4) production in various plant tissues. The present study was made to determine the step in the C(2)H(4) biosynthetic pathway (methionine --> S-adenosylmethionine [SAM] --> 1-aminocyclopropane-1-carboxylic acid [ACC] --> C(2)H(4)) at which these treatments exert their inhibitory effect. In mung bean hypocotyls the dose-inhibition curves for the effect of DNP on auxin-dependent C(2)H(4) production (in which auxin exerts its effect by stimulating the conversion of SAM to ACC) and on ACC-dependent C(2)H(4) production (in which ACC is directly utilized as precursor) were similar. It was concluded, therefore, that DNP at low concentrations (below 50 micromolar) exerted its effect primarily on the conversion of ACC to C(2)H(4), a step which is common to both systems. This view was further substantiated by quantitative analysis of the intermediates in the biosynthetic sequence. DNP exerted little influence on the content of SAM but caused a significant increase in the ACC content and marked inhibition in C(2)H(4) production, indicating that the conversion of ACC to C(2)H(4) is the crossover point. At higher concentrations (above 100 micromolar), DNP inhibited the conversion of methionine to ACC and to C(2)H(4), and this effect could be attributed to the inhibition of SAM synthesis.The optimal temperature for maximal C(2)H(4) production by apple tissue and mung bean hypocotyl is about 30 C. An increase in temperature to 35 C caused an accumulation of endogenous ACC, whereas C(2)H(4) production was greatly reduced. These results suggest that the conversion of ACC to C(2)H(4) is highly vulnerable to high temperature inhibition.