Abstract

This research was undertaken to study the mode of action of low O2 in delaying ripening of avocado fruit. Hass avocado fruits were treated with propylene in various O2 concentrations (1%, 2%, 5%, and 21%) at 20°C and ethylene production and ripening of the fruit were followed by measuring accumulation of ACC, EFE activity and the polygalacturonase (PG) and cellulase (Cx) activities respectively. Low O2 delayed the softening of propylene treated fruits and prevented the rise in EFE activity. Furthermore, low O2 suppressed the rise in PG and Cx activities. Gel electrophoresis and western blot analysis showed that the levels of PG and Cx proteins, were drastically reduced in fruits held in low O2 (1% and 2%).

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