Abstract
The purpose of this study was to investigate the effect of curcumin-treated Herpes simplex virus-1 (HSV-1) and Herpes simplex virus-2 (HSV-2) virions in cultured Vero cells. Previous studies have indicated that curcumin, a polyphenol extracted from the plant Curcuma longa, has demonstrated antiviral properties against a variety of viruses. After establishing the maximum non-cytotoxic concentrations of curcumin on Vero cells, HSV-1 and HSV-2 virions were treated with varying concentrations of curcumin. The effect on infectivity was determined by antiviral assays, using WST-1, plaque assays, adsorption and penetration assays. Treating HSV-1 and HSV-2 viruses with curcumin, at a concentration of 30 μM, reduces the production of infectious HSV-1 and HSV-2 virions in cultured Vero cells by interfering with the adsorption process. These results support the potential of curcumin to be used as a therapeutic agent to reduce the transmission of HSV-1 and HSV-2.
Highlights
Herpes simplex virus 1 (HSV-1) and Herpes simplex virus 2 (HSV-2) are transferred among humans via an oral or sexual route [1] [2]
The purpose of this study is to investigate the effect of curcumin-treated HSV-1 and HSV-2 virions in cultured Vero cells
The cell viability was determined by using trypan blue and hemacytometer direct cell count to detect the effect of curcumin on Vero cells
Summary
Herpes simplex virus 1 (HSV-1) and Herpes simplex virus 2 (HSV-2) (family Herpesviridae, subfamily Alphaherpesvirinae) are transferred among humans via an oral or sexual route [1] [2]. HSV virions must attach to cellular receptors, and fuse their envelopes with host cell plasma membranes [3]-[5]. This process of adsorption is followed by penetration and transport of the encapsidated DNA genome to the nuclear pore, where the viral DNA is released [6]. Following infection of epithelial cells, HSV is able to migrate. (2016) Inhibition of Curcumin-Treated Herpes Simplex Virus 1 and 2 in Vero Cells. Periodic outbreaks occur due to reactivation, resulting in viral shedding from lesions [7]
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