Abstract
ABSTRACTThe effects of sodium acid pyrophosphate (SAPP), sodium chloride (NaCl) and/or potassium sorbate (PS) on the growth from heat‐activated spores of three individual strains or a mixture of ten strains of Clostridium botulinum in peptone‐yeast extract‐glucose broth at pH 5.55 or 5.85 were measured spectrophotometricalty at A630nm. Growth ratios (GR = treatment/control) based on time to reach A630= 0.35 or 0.04 were calculated and used to compare effects of additives on strains. SAPP, NaCl, PS, and pH exhibited independent significant main effects (p≦0.01) on delaying growth in most C. botulinum strains tested. Combinations of additives without NaCl consistently caused an increase in the GR and an increase in organism sensitivity to additives in the medium. Treatments containing SAPP (0.2 or 0.4%) and PS (0.13 or 0.26%) were more effective for delaying growth than other formulations tested.
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