Inhibition of cell proliferation in gastric cancer cell lines on exposure to rubriflordilactone A

Shang-Jin Peng,Jue-Wei Chen

doi:10.3329/bjp.v11i1.23772

Abstract

<p class="Abstract">The present study investigates the effect of rubriflordilactone A on the viability and its underlying mechanism in gastric cancer cell lines (SNU-1 and SNU-5) and normal gastric epithelial cell line (GES‑1). Incubation of the gastric cancer and non cancer cell lines in acidic media led to reduction in the viability of the non cancer cells without any effect on cancer cells. Apoptosis in SNU-1 and SNU-5 cells was induced on exposure to rubriflordilactone A after 48 hours compared to the control cells (p&lt;0.01). The percentage of apoptosis in SNU-1 and SNU-5 cells on exposure to rubriflordilactone A was 79.3 ± 4.7 and 74.0 ± 5.1, respectively after 48 hours. Exposure of SNU-1 and SNU-5 cancer cell lines to rubriflordilactone A at a concentration of 10 μM in media with acidic pH decreased phosphorylation of ERK ½. The similar reduction was caused by ERK 1/2 phosphorylation inhibition, PD98059. Thus rubriflordilactone A reduces viability of gastric cancer cell lines by inducing apoptosis through the reduction of ERK 1/2 phosphorylation.</p><p> </p>

Highlights

Gastric cancer is the most commonly detected and one of the leading cause of cancer deaths throughout the globe (Ferlay et al, 2010; Jemal et al, 2010)
Incubation of the SNU-1 and SNU-5 cell lines and GES‐1 cell line in the media with acidic pH revealed a marked decrease in the viability of non-cancer cell line compared to two cancer cell lines
Exposure of the gastric cancer cell lines, SNU-1 and SNU-5 cultured in acid media to 10 μM concentration of rubriflordilactone A for 24 hours led to a significant reduction in the viability of these cell lines (Figure 3)

Summary

Introduction

Gastric cancer is the most commonly detected and one of the leading cause of cancer deaths throughout the globe (Ferlay et al, 2010; Jemal et al, 2010). In Asian countries the rate of gastric cancer deaths is higher at present compared to the African countries (Jemal et al, 2011; Sun et al, 2004). Rubriflordilactones A (Figure 1) from isolated from the Schisandra rubriflora that has been widely used in Chinese herbal medicine (Xiao et al, 2006). It exhibits promising anti-HIV activity and antitumor activity (Xiao et al, 2006). The effect of rubriflordilactone A on the viability and induction of apoptosis in the gastric cancer cells is for the first time investigated. Taking clue from the literature that plant derived natural products play an important role in the inhibition of various types of cancers (Ji et al, 2008; Majewska et al, 2006; Zhong et al, 2003; Zhong et al, 2004; Abdullaev and Espinosa‐Aguirre, 2004; Das et al, 2010)

Methods

Results

Conclusion