Inhibition of Candida spp. growth in Vaginitis women using a Chitosan-silver nanocomposite

Abstract

Yeasts were isolated from vaginitis women. A total of 50 Samples of vaginal swabs was collected from Al-Hakim and Al-Furat Hospital in Al-Najaf province. The number of samples that appeared growth is 40 samples while the samples that not appeared growth of yeasts is 10 samples. Candida species are divided into two groups were isolated that are C.albicans and C. kuseie. Candida is an opportunistic pathogen and caused vulvovaginal infections. C. albicanis was found more frequently than C. krusei with (44,11)% respectively. Candida spp. with cream color cultivated easily at 48 hours on sabouraud dextrose agar, the colonies are smooth and glitter. Candida spp identifying with utilizing chrom agar that is regard exhibiting a difference agar of colonies, C.albicans be a characteristic of by slight green color, become smooth colonies while C. crazy pink and soft peripheral at a temperature (37)0C after 2 days of incubation. Chitosan -silver nanocamposite is used to inhibit growth of Candida with high efficiency .The percentage inhibition of C. albicanis is 54% in the concentration of 50 ppm and 92% in 100ppm, while in C. krusi the percentage of inhibition is 40% in 50ppm while 80% in 100 ppm. 150ppm of Chitosan-silver nanocomposite causes complete inhibition growth of C. albicans and C. krusei.