Abstract
AA861, a specific 5-lipoxygenase inhibitor, inhibited bovine lymphocyte response to phytohemagglutinin (PHA). Mitogen-stimulated cultures of mononuclear cells produced leukotriene B 4 (LTB 4) in 24 hours. The production of LTB 4 was completely inhibited by concentrations of AA861 that inhibited mitogen-induced 3H-thymidine incorporation. The inhibition of lymphocyte proliferation was totally reversed by LTB 4 but not by leukotriene C 4 or leukotriene D 4. The inhibition of interleukin-2 (IL-2) production by AA861 was also completely reversed by addition of exogenous LTB 4 to lymphocyte cultures. Thus, endogenous LTB 4 production appeared to be necessary for PHA-induced IL-2 production and lymphocyte proliferation.
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