Abstract
The inhibition of biocorrosion of steel coupons by sulphate reducing bacteria (SRB) and Iron oxidizing bacteria (IOB) using Aloe vera ( Aloe barbadensis ) extract was tested. The water sample revealed a heterotrophic bacterial count of 1.7x103 cfu/ml for the sulphate reducing bacteria and 4.1x103 cfu/ml for the Iron oxidizing bacteria. Biocorrosion inhibition test using Aloe vera extract was prepared with 50 % w/v stock solution at 10 %, 20 %, 40 % and 80 % (v/v) concentrations. An increase in acidity was observed in the fresh post gate medium after 7 days to 6.6 with a slight drop to 6.5 after 28 days in the setup with SRB. In contrast, the pH of the setups with IOB showed an increase in alkalinity from 6.8 in fresh Winogradsky medium to 7.4 after 7 days. The conductivity of SRB setup increased from 3150 μS/cm to 27100 μS/cm after 7 days and decreased to 200 μS/cm after 28 days. On the other hand, the IOB setup showed an increase in conductivity from 100 μS/cm to 2820 μS/cm after 28 days. The surfaces of the metal coupon used in the biocorrosion testing were slimy to touch, indicating possible biofilm production. The average corrosion rate from day 7 to day 28 for IOB and SRB are 6.606 mpy and 12.48 mpy respectively. The 80 % v/v concentration of Aloe vera extract gave the highest inhibition efficiency, ranging from 89 %-100 % for IOB and 66 %- 100 % for SRB. Aloe vera extract inhibited corrosion of steel coupons mediated by aerobic iron oxidizing bacteria and anaerobic sulphate reducing bacteria. Keywords: Aloe Vera extract, biocorrosion, metal coupons, oxidizing bacteria, reducing bacteria
Highlights
ABSTRACT; The inhibition of biocorrosion of steel coupons by sulphate reducing bacteria (SRB) and Iron oxidizing bacteria (IOB) using Aloe vera (Aloe barbadensis) extract was tested
A looful of sulphate reducing bacteria was suspended in fresh Postgate broth and another set up containing Postgate broth and 10 %, 20 %, 40 % and 80 % Aloe vera extract respectively
The biocorrosion of Steel coupons by SRB and Iron oxidizing bacteria using Aloe vera extract as a green inhibitor of biocorrosion was evaluated
Summary
Preparation of steel Coupon Specimen; The steel bar used in this experiment was cut mechanically into strips of size 1.8 cm × 1 cm × 0.2 cm, polished with fine grade emery papers, washed, sterilized in an autoclave and weighed using electronic balance. Preparation of Stock solution The stock solution was prepared by weighing 8g of the Aloe vera extract in 16 ml of deionised water This gave a concentration of 50 % w/v. A looful of sulphate reducing bacteria was suspended in fresh Postgate broth and another set up containing Postgate broth and 10 %, 20 %, 40 % and 80 % Aloe vera extract respectively. Forty (40) preweighed metal coupons (Steel coupons) labelled S1 S40 were placed in pairs inside Bijou bottles containing both set up and incubated anaerobically at 300C for 7 days. The same procedure was repeated for a 7 day interval thrice (14, 21 and 28 days) The controls for both Iron oxidizing bacteria and Sulphate reducing bacteria set up comprised uninoculated sterile media. Atomic absorption spectrophotometry was used to analyze the Fe2+ of the set ups
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