Abstract
The rapid cessation of DNA synthesis induced in E . coli K12 Hfr G6 his − DNA-C − by histidine deprivation, reported by Suit, Matney, Doudney, and Billen (1964), has been confirmed. Evidence is prevented that the ability of this Hfr to participate as a genetic donor in bacterial mating is drastically reduced by histidine deprivation during mating. The conflicting results previously reported by Suit et al. are accounted for in terms of histidine supplementation of donor cells by his + recipient cells.
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