Abstract
Microbial adhesion and formation of biofilms cause a serious problem in several areas including but not limited to food spoilage, industrial corrosion and nosocomial infections. These microbial biofilms pose a serious threat to human health since microbial communities in the biofilm matrix are protected with exopolymeric substances and difficult to eradicate with antibiotics. Hence, the prevention of microbial adhesion followed by biofilm formation is one of the promising strategies to prevent these consequences. The attachment of antimicrobial agents, coatings of nanomaterials and synthesis of hybrid materials are widely used approach to develop surfaces having potential to hinder bacterial adhesion and biofilm formation. In this study, epigallocatechin gallate (EGCG) is attached on p(HEMA-co-GMA) membranes to prevent the bacterial colonization. The attachment of EGCG to membranes was proved by Fourier-transform infrared spectroscopy (FT-IR). The synthesized membrane showed porous structure (SEM), and desirable swelling degree, which are ideal when it comes to the application in biotechnology and biomedicine. Furthermore, EGCG attached membrane showed significant potential to prevent the microbial colonization on the surface. The obtained results suggest that EGCG attached polymer could be used as an alternative approach to prevent the microbial colonization on the biomedical surfaces, food processing equipment as well as development of microbial resistant food packaging systems.
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