Abstract
The effects of cell-growth regulating agents of apolipoprotein(a) [apo(a)] synthesis were examined in human hepatocarcinoma cell lines. Among four cell lines, apo(a) can be detected in three cell lines, SK-HEP-1, Hep 3B and Chang cells, but can not be detected in Hep G2 cells at the same protein concentration. Particularly, SK-HEP-1 cells synthesized the highest level among the examined cell lines. Two cell lines, SK-HEP-1 and Hep 3B cells were treated with several cell-growth regulating agents; dimethyl sulfoxide, all-trans retinoic acid, interleukin-6, phorbol 12-myristate 13-acetate (PMA) and sodium butyrate. Culture media were harvested to determine the apo(a) level which was produced from these cells. Dimethyl sulfoxide and retinoic acid inhibited apo(a) synthesis dosedependently. In both cell lines, maximum inhibitions induced by dimethyl sulfoxide and retinoic acid were observed at 2.0% and 2.0 μM, respectively. PMA increased apo(a) synthesis in both cell lines. Interleukin-6 suppressed apo(a) synthesis in dose-dependent manner and completely blocked at 2,000 U/㎖ in Hep 3B cells, while there was no changes in SK-HEP-1 cells.
Highlights
Lipoprotein(a) [Lp(a)] is composed of a low density lipoprotein (LDL) particle and a characteristic protein component called apolipoprotein(a) [apo(a)] which is linked to apolipoprotein B-100 by disulfide bond
Demonstration of apo(a) synthesis in several human hepatocarcinoma cell lines To test whether available hepatocarcinoma cell lines can produce apo(a), the level of apo(a) in the culture media was determined using monoclonal antibody to human Lp(a)
SK-HEP-1 cells produced a high level of apo(a)
Summary
Lipoprotein(a) [Lp(a)] is composed of a low density lipoprotein (LDL) particle and a characteristic protein component called apolipoprotein(a) [apo(a)] which is linked to apolipoprotein B-100 (apo B-100) by disulfide bond. Demonstration of apo(a) synthesis in several human hepatocarcinoma cell lines To test whether available hepatocarcinoma cell lines can produce apo(a), the level of apo(a) in the culture media was determined using monoclonal antibody to human Lp(a). It was suggested that 180 kDa component is the apo(a) expressed in human hepatocarcinoma cells, which was derived from a minimum number of kringle 4-repeats and lower glycosylation.
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