Inhibition of amino acid accumulation in slices of rat kidney cortex by diamide

Abstract

1. 1.The oxidizing agent diazenedicarboxylic acid bis-( N, N-dimethylamide), common name diamide, has been used to lower the concentration of GSH in rat kidney cortex slices. 2. 2.The ability to accumulate l-amino acids against a concentration gradient was decreased in diamide-treated slices. 3. 3.Studies with six different amino acids have shown that this inhibition of amino acid accumulation is not specific for one amino acid or one group of related amino acids. 4. 4.The addition of exogenous GSH to slices that have been treated with diamide leads to a significant recovery of the ability to concentrate amino acids. 5. 5.Dithiothreitol and mercaptoethanol also reverse the effect of diamide, but are less effective than GSH.