INHIBITION OF ADHESION OF UROPATHOGENIC ESCHERICHIA COLI TO CANINE AND FELINE UROEPITHELIAL CELLS BY AN EXTRACT FROM CRANBERRY

Gilles Mayot,Camille Secher,And Patrick Di Martino

doi:10.15414/jmbfs.2018.7.4.404-406

Gilles Mayot, Camille Secher + Show 1 more

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https://doi.org/10.15414/jmbfs.2018.7.4.404-406

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Abstract

Uropathogenic Escherichia coli (UPEC) is the main infectious agent of urinary tract infections (UTI) in humans, dogs and cats. Dietary consumption of cranberries is thought to be associated with prevention of UTI in humans based on decreased adhesion of UPEC to uroepithelial cells. The present study evaluated the impact of cranberry extract addition on the attachment of UPEC to canine Madin-Darby Canine Kidney and Crandell-Rees Feline Kidney uroepithelial cells. When the extract was present during bacterial growth or only during adhesion tests, a dose-dependent decrease of UPEC adhesion to all cell types was observed. Bacterial growth was weakly decreased only in the presence of the highest concentration of cranberry extract showing that the anti-adherence effect did not require a bacterial growth inhibitory effect. In conclusion, the addition of cranberry extract has preventive effects on the in vitro bacterial attachment to canine and feline uroepithelial cells in a dose dependent way.

Highlights

Bacterial urinary tract infections (UTI) are common in dogs but less common in cats (Litster et al, 2011; Thompson et al, 2011)
Ascending uropathogenic E. coli (UPEC) strains usually have a particular repertoire of virulence genes including genes encoding for adherence factors such as fim, pap, sfa (S fimbriae), and genes encoding for exotoxins such as hly ( -hemolysin), and cnf1
Some E. coli isolates from humans, dogs, and cats have close similarity in the genomic backbone and virulence genotype being sometimes qualified as uropathogenic clones (Johnson et al, 2000; Féria et al, 2001; Johnson et al, 2008a; Johnson et al, 2008b)

Summary

Introduction

Bacterial urinary tract infections (UTI) are common in dogs but less common in cats (Litster et al, 2011; Thompson et al, 2011). There is a need for studies evaluating the use of cranberry to prevent urinary tract infections in dogs and cats based on the inhibition of bacterial adhesion to canine and feline uroepithelial cells. Adherence to human T24, canine MDCK, and feline CRFK urinary epithelial cells of Escherichia coli G1473: presence of cranberry during bacterial growth and absence of cranberry during the adherence test (condition 1).

Results

Conclusion