Inhibition de la multiplication virale á l'aide d'acides ribonuclêiques chimiquement modifiès: III Influence de la nature et du taux des modifications

Abstract

Inhibition of viral multiplication by chemically modified ribonucleic acids III. Influence of the nature and the extent of the chemical modifications The multiplication of an Arbovirus (virus Sindbis) on fibroblasts of chicken embryos in the presence of RNA extracted from chicken embryos and modified by aarious chemical reagents was studied. All these chemically modified RNA's induce on inhibition of the viral multiplication. This effect is dependent both on the quantity vf RNA introduced into the cell culture medium and on the level of the chemical modification. In the case of several modifications such as methylation, allylation, deamination by nitrous acid, chloruration or iodination, an increase in the percentage of modified bases gives rise to an increase in the inhibition of the viral multiplication (90–100%). On the contrary, when the RNA is modified by oxidation with mono-perphthalic acid, the increase in the percentage of modified bases produces a decrease in the level of inhibition. This difference is discussed in relation with the integrity of the primary and secondary structure of the RNA's.