Abstract
Applied diamines and polyamines inhibited the incorporation of radioactively labeled leucine and uridine into trichloroacetic acid-insoluble material in apple (Malus domestica Borkh, cv Golden Delicious) fruit tissue. The inhibitory effect was in general more pronounced with the higher molecular weight amines. Putrescine at 5 millimolar inhibited leucine incorporation by 37% and uridine by 44%. Spermidine and spermine at the same concentration inhibited uridine incorporation by 60%. The polyamines at concentrations between 0.1 and 1.0 millimolar inhibited leucine incorporation by 55 to 90%. The inhibitory effect of 0.1 to 10 millimolar polyamines on dark- and wound-induced senescence or ethylene production, is discussed in the light of interference with macromolecular synthesis.
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