Inhibition by cyclic AMP and dibutyryl cyclic AMP of transport of organic acids in kidney cortex

Abstract

1. 1.|Cyclic adenosine 3′,5′-monophosphate and N 6-2′- O-dibutyryl cyclic adenosine 3′,5′-monophosphate decrease the initial entry rate and the steady-state uptake of p-aminohippurate and uric acid by rabbit kidney cortex slices. 2. 2.| N 6-2′- O-Dibutyryl adenosine 3′-5′-monophosphate inhibits the tubular transport of p-aminohippurate competitively. 3. 3.|Isoproterenol, known to increase cyclic nucleotide concentration of the cortical tubules by activation of adenyl cyclase, decreases p-aminohippurate transport. Antidiuretic hormone which is known to stimulate only medullary adenyl cyclase has no effect on p-amino-hippurate uptake by cortical slices. 4. 4.|Theophylline, which inhibits cyclic nucleotide phosphodiesterase and, therefore, enhances the cellular accumulation of endogenous cyclic nucleotide, depresses p-aminohippurate transport.