Inhibition by cyclic AMP and dibutyryl cyclic AMP of aggregation of embryonic quail liver cells in culture

Abstract

The effects of cAMP and DB cAMP on the aggregation of dissociated embryonic quail liver cells were examined in rotation-mediated cell culture. Both cAMP and DB cAMP had concentration-dependent inhibitory effects on the aggregation of cells. At a concentration of 0.6 mg/ml of cAMP, aggregates formed after 24 h and 48 h of rotation culture had half the mean diameter of those obtained in respective control cultures. DB cAMP had stronger inhibitory effects than cAMP at the same concentrations. When aggregates formed after 24 h in media containing cAMP at various concentrations were transferred to normal medium and cultured for a further 24 h, they recovered their cohesiveness to form larger aggregates. By contrast, aggregates cultured for 24 h with DB cAMP lost almost completely their aggregability in further cultivation in normal medium.