Abstract
The effects of incorporating bile salts alone or bile salts and bran in the diet were investigated in 1,2-dimethylhydrazine (DMH)-induced intestinal carcinogenesis in rats. Male Sprague-Dawley albino rats were divided into the following groups: Group I, control; Group II, 0.5% mixed bile salts; Group III, 30 mg/kg DMH (10 weekly oral doses); Group IV, bile salts and DMH; and Group V, bile salts, 20% wheat bran and DMH. The bile salts and bran were added to a standard basal diet. The numbers of grossly observable colonic tumors/rat in Groups III, IV, and V were 10.8, 8.4, and 11.6, respectively. However, microscopic classification of these tumors revealed 37 46 malignant tumors in Group IV when compared to Group III ( 28 68 ) and Group V ( 43 77 ). Thus bile salts were cocarcinogenic but not cotumorigenic, that is, bile salts caused no increase in the number of grossly observable tumors, but rather potentiates the formation of malignant tumors. Bile salts alone increased the labeling index of epithelial cell nuclei in the lower third of the colonic cryts, while in combination with DMH, they increased the number of cells/crypt. Further, addition of bile steroids significantly increased the incidence and number of malignant duodenal tumors. No significant differences in the fecal excretion of acidic steroids were observed between Groups II, IV, and V.
Published Version
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