Abstract
A multienzyme complex consisting of DNA polymerase and several DNA precursor-synthesizing enzymes was solubilized by gentle lysis of cultured human cells. This complex channelled the distal precursor [ 3H]dTMP into DNA. The patterns of inhibition of the complex by aphidicolin and dideoxythymidine triphopshate (ddTTP) suggested that the complex contained the replicative DNA polymerase, polymerase α. Inhibition by ddTTP was competitive with dTTP. This was exploited to estimate the effective concentration of [ 3H]dTTP at the site of DNA synthesis during channelling of [ 3H]dTMP into DNA. The estimated concentration (about 50 μM) was so high as to suggest that the solubilized complex was able to functionally compartmentalize DNA precursors.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
