Abstract
Antisense oligodeoxynucleotides were used to knock down plasma membrane Ca 2+ ATPase, and the role of plasma membrane Ca 2+ ATPase was investigated in human aortic endothelial (HAE) cells. The peak of thapsigargin-evoked intracellular Ca 2+ concentration ([Ca 2+] i) was higher in antisense-treated than in untreated cells, but the declining time course was unaffected by the antisense treatment. The declining time was prolonged in both antisense-treated and untreated cells by reducing external Na +, but the prolongation was more marked in the antisense-treated cells. These results provide the evidence of a functional role of plasma membrane Ca 2+ ATPase, although other mechanisms including Na +/Ca 2+ exchange may play the primary role in regulating [Ca 2+] i.
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