Abstract
BackgroundKeloid is one kind of benign skin disease caused by hyperplasia of fibroblasts and collagen fibrils. It is refractory due to the lack of an effective treatment at present, which puts pressure on seeking a new therapeutic regimen. Mesenchymal stem cells (MSCs) from fetal skin are considered to play a crucial role in scarless healing. Nevertheless, the efficacy of them in keloid disorders remains poorly understood.MethodsKeloid fibroblasts (KFs), human adult dermal fibroblasts (ADFs), and human fetal dermal mesenchymal stem cells (FDMSCs) were isolated to single cells and cultured in Dulbecco’s modified Eagle’s medium (DMEM). ADFs and FDMSCs were used to generate ADF-conditioned medium (A-CM) and FDMSC-conditioned medium (F-CM). The effects of A-CM and F-CM on KFs were tested using MTT assay, BrdU assay, TUNEL assay, quantitative polymerase chain reaction, Western blot, and annexin V-FITC/PI binding assay,.ResultsFDMSCs inhibited the bioactivity of KFs, downregulated the expression of the antiapoptotic protein BCL-2, and upregulated the expression of the proapoptotic protein BAX of KFs by secreting some soluble substances, thus accelerating the apoptosis of KFs.ConclusionF-CM induces apoptosis of KFs, providing a novel treatment strategy for keloid disorders.
Highlights
Keloid is one kind of benign skin disease caused by hyperplasia of fibroblasts and collagen fibrils
We investigated the effects of fetal dermal Mesenchymal stem cell (MSC) (FDMSC)-conditioned medium (F-CM) on keloid fibroblasts (KFs)
Our studies revealed that FDMSCs could induce the apoptosis of Keloid fibroblast (KF) by secreting some soluble substance and we further verify the feasibility of new treatments
Summary
Keloid is one kind of benign skin disease caused by hyperplasia of fibroblasts and collagen fibrils. It is refractory due to the lack of an effective treatment at present, which puts pressure on seeking a new therapeutic regimen. Mesenchymal stem cells (MSCs) from fetal skin are considered to play a crucial role in scarless healing. As one kind of benign skin disease, keloid is caused by hyperplasia of fibroblasts and collagen fibrils [1]. Keloid is a clinically frequently occurring disease, it is refractory due to the lack of an effective treatment. There is a pressing need to seek a new therapeutic regimen. Recent research has indicated that mesenchymal stem cells (MSCs) play significant roles in scarless wound healing and tissue regeneration [3,4,5]. Fetal skin cells are thought to play key roles in fetal scarless healing [6, 7]
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