Abstract
Abscisic acid (ABA) treatments induce in barley leaf fragments an increase in proline with a maximum at 10 −4 M ABA. This effect is suppressed by the presence of fusicoccin (FC). FC induces an acidification on the incubation medium paralleled by an increase in cell sap pH; both of these effects of FC are significantly antagonized by the simultaneous addition of ABA. The antagonism between FC and ABA on the proline accumulation process and on the external pH changes suggests that both of these substances can influence H + secretion, presumably by acting on the proton pump, and that the consequent changes in intracellular pH might affect some metabolic process and thus influence proline metabolism.
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