Inhibiting Copper Amine Oxidase Using L-Aminoguanidine Induces Cultivar and Age-Dependent Alterations of Polyamine Catabolism in Tomato Seedlings

Ágnes Szepesi,László Bakacsy,Henrietta Kovács,Árpád Szilágyi,Zoltán Márton Köhler

doi:10.3390/agriculture12020274

Abstract

Many effects of polyamines (PAs) are well known in plant developmental processes; however, the significance of their catabolism is not well understood. Copper amine oxidase (CuAO) is involved in the degradation of diamine putrescine (Put). The genotype dependence and time-dependent effects of this enzyme are rarely examined, so this study aims to discover the role of CuAO in tomato genotypes in different stages of seedling development. Exogenously applied L-aminoguanidine (AG), a CuAO inhibitor, was used to decrease the activity of CuAOs. Based on our results, it can be concluded that there is a genotype dependence of Put degradation, and AG treatment caused a long-term shift of PA catabolism by changing the activities of polyamine oxidase (PAO), catalyzing the degradation of higher PAs. Our results demonstrate that the modification of PA catabolism could have long-term results in polyamine metabolism in different tomato genotypes.

Highlights

The tomato (Solanum lycopersicum Mill.L.) is the second most important and widely consumed vegetable crop plant next to the potato in the world [1]
Germination Time Courses of Tomato Seeds We examined the germination time courses of the tomato genotypes
Free polyamine Contents of Tomato Seedlings at 10 Days after Germination We investigated how applied Laminoguanidine (AG) treatment could induce long-term alterations in tomato genotypes

Summary

Introduction

The tomato (Solanum lycopersicum Mill.L.) is the second most important and widely consumed vegetable crop plant next to the potato in the world [1]. Its importance is for the food industry and in human medicine. The biodiversity of tomatoes is extremely high because of the huge numbers of genotypes [2]. Despite the enormous amount of data and the number of studies that have been generated about the tomato stress responses, the genotype-dependent growth responses are rarely examined. Polyamines are essential polycationic molecules contributing to the growth and development of plants. Their metabolism plays a significant role in plant cells acting similar to a hub [3,4,5]

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