Inhibiting cell migration and cell invasion by silencing the transcription factor ETS-1 in human bladder cancer.

Li Liu,Chengle Zhuang,Jianfa Li,Wen Xu,Junhao Lin,Weiren Huang,Xintao Zhang,Qiaoxia Zhang,Yonghao Zhan,Xiaojuan Sun,Guoping Zhao,Muqi Lin,Mingwei Chen,Xing Fu,Yuchen Liu,Hanwei Wu

doi:10.18632/oncotarget.7192

Abstract

As one of the members of the ETS gene family, the transcription factor v-ets avian erythroblastosis virus E26 oncogene homolog 1 (ETS-1) plays key role in the regulation of physiological processes in normal cells and tumors. In this study, we aimed to investigate the relationship between the transcription factor ETS-1 and malignant phenotypes of bladder cancer. We demonstrated that ETS-1 was up-regulated in human bladder cancer tissue compared to paired normal bladder tissue. In order to evaluate the functional role of ETS-1 in human bladder cancer, vectors expressing ETS-1 shRNA and ETS-1 protein were constructed in vitro and transfected into the human bladder cancer T24 and 5637 cells. Our results showed that the transcription factor ETS-1 could promote cell migration and cell invasion in human bladder cancer, without affecting cell proliferation and apoptosis. In conclusion, ETS-1 plays oncogenic roles through inducing cell migration and invasion in human bladder cancer, and it can be used as a therapeutic target for treating human bladder cancer.

Highlights

Human bladder cancer is one of the most common cancers worldwide [1]
ETS-1expression level was increased in bladder cancer
The results indicated that ETS-1 expression level was associated with the depth of invasion in these patients with bladder cancer, but it has no relationship with the age, the gender and the grade. These results suggested that ETS-1overexpression may be related to bladder cancer invasion

Summary

Introduction

Human bladder cancer is one of the most common cancers worldwide [1]. Methods for early diagnosis of bladder cancer and more suitable surgeries were emerged, the mortality rate has not been decreased effectively [2]. One of these important reasons is that the potential mechanisms causing cancer progress are still poorly understood. Searching novel reliable biomarkers and revealing the molecular mechanism of bladder cancer for developing effective therapy are necessary. The functional character of ETS-1 in human bladder cancer is still unclear. We aimed to study the expression pattern of ETS-1 and to determine its functional role in human bladder cancer

Objectives

Methods

Results

Conclusion