Abstract
Based on efficient in vitro culture and regeneration systems, we have developed reliable protocols for direct DNA uptake into barley, triticale, oat, maize and wheat. Our present experiments are aiming at the transfer of genes of agronomic interest. However, transgenic crop plants will only be of value if their engineered phenotype is faithfully transmitted through subsequent generations in a predictable manner. Therefore, we investigated the inheritance and expression of transgenes at the molecular level in transgenic wheat plants. In most of our independent lines, we observed a mendelian inheritance pattern. Gene inactivation was observed in a few lines containing multicopy-integrations of the transgene. We investigated non-mendelian inheritance in a single-copy transgenic wheat plant line. Transmission rate amounted 1:1 instead of the expected 3:1-ratio. Furthermore, non of the analyzed plants was homozygous for the transgene. Several sources for these phenonemons were investigated in detail.
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