Inheritance of Total and Individual Glucosinolate Contents in Seeds of Winter Oilseed Rape (Brassica napus L.)

Abstract

AbstractGenetic analyses were carried out with B. napus lines that differed in total content of individual glucosinolates (GSLs), as well as in their profile. Inheritance of total GSL content was studied with a complete F1 diallel mating of eight doubled haploid (DH) lines (5–120/μmol GSL/g dry seed) as well as with the segregating populations of two crosses between DH lines. Most of the genetic variability was caused by gca effects; heterosis was not important; heritability of GSL content was high (h2b, = 0.95, h2n= 0.87). For GSL contents below 20/μmol/g seed, heritability values reached h2b, = 0.69 and h2n, = 0.66. Low total GSL content was found to be controlled by 4–5 recessive genes with additive gene action. Alkenyl GSL profiles studied in F2 from resynthesized rapeseed lines were determined by four loci, two that are responsible for the elongation of the butenyl to pentenyl GSLs and two that are responsible for hydroxylation of alkenyl GSLs. Initial genetic studies of indolyl GSL contents from 0.1–4.5/μmol/g show that two or three genes may be involved. The frequency distribution of F2 phenotypes was virtually continuous due to both segregational and environmental variation. Genotypes with low alkenyl and low indolyl GSL contents were selected after crossing parents with low alkenyl/high indolyl and high alkenyl/low indolyl GSL content. The alkenyl GSL profiles are discussed with regard to the parental diploid species B. oleracea and B. campestris.