Inheritance of spot blotch resistance in barley (Hordeum vulgare L.)

Sarvjeet Singh,Neha Joshi,Harvinder Singh,Shiv Kumar,Baljit Singh,Achla Sharma,Adnan Al-Yassin,Madhu Meeta,Pooja Grover

doi:10.4141/cjps2013-153

Abstract

Singh, S., Singh, H., Sharma, A., Meeta, M., Singh, B., Joshi, N., Grover, P., Al-Yassin, A. and Kumar, S. 2014. Inheritance of spot blotch resistance in barley (Hordeum vulgare L.). Can. J. Plant Sci. 94: 1203–1209. Spot blotch, caused by Bipolaris sorokiniana, is a major disease of barley hampering its commercial production in many parts of the world. Growing resistant cultivars is the most effective, economical and eco-friendly approach to control the disease. To understand the inheritance of resistance, F1, F2 and F3 generations of two crosses, involving susceptible (PL426 and RD2503) and resistant (BL9 and BL10) parents were studied. The susceptibility of F1 plants and a ratio of 13 susceptible:3 resistant in F2 populations in both crosses indicated that the reaction to spot blotch is hypostatic and is governed by two genes with an epistatic/inhibitory effect of first on the second one. The resistant reaction appeared due to the presence of dominant allele of the second gene. The first gene in dominant homozygous or heterozygous condition had an inhibitory effect over the second gene. The inheritance pattern was confirmed from the segregation pattern of F3 progenies of both the crosses. One hundred-fifty F2:6 recombinant inbred lines (RIL) derived from PL426/BL10 cross gave a good fit to the ratio of 1 resistant:3 susceptible lines under artificial and natural epiphytotic conditions. Recovery of transgressive segregants in the RIL population indicated the presence of some modifiers or minor genes. Test of allelism involving susceptible (PL426)×susceptible (RD2503) and resistant (BL9)×resistant (BL10) parents revealed that both the susceptible parents as well as resistant parents were allelic to each other.

Highlights

Singh, S., Singh, H., Sharma, A., Meeta, M., Singh, B., Joshi, N., Grover, P., Al-Yassin, A. et Kumar, S. 2014
Understanding the inheritance of resistance to disease is valuable for planning crosses in breeding programs, identifying resistance genes and developing genetic markers to assist in selection
PL426 and RD2503 are susceptible to spot blotch disease and are still widely grown in different agroclimatic regions in India, while BL9 and BL10 are the advanced breeding lines from ICARDA (International Centre for Agricultural Research in the Dry Areas, Syria) with high spot blotch resistance but poor adaptation to the Indian conditions

Summary

Introduction

S., Singh, H., Sharma, A., Meeta, M., Singh, B., Joshi, N., Grover, P., Al-Yassin, A. et Kumar, S. 2014. La sensibilitedes plants de la F1 et le rapport de 13 plants sensibles pour 3 resistants dans la F2 pour les deux croisements revelent que la reaction ala tache helminthosporienne est hypostatique et regie par deux genes, le premier ayant un effet epistatique/inhibiteur sur le second. This disease can cause 10 to 100% yield losses in susceptible cultivars besides causing deterioration of grain quality (Clark 1979; van Leur 1991; Mathre 1997). Comprehensive and clear information with respect to inheritance of spot blotch resistance is required to design suitable breeding strategies to enhance resistance of high yielding barley cultivars. The objective of this research was to study the inheritance of spot blotch resistance

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