Inheritance of Rugose Leaf in Caladium and Genetic Relationships with Leaf Shape, Main Vein Color, and Leaf Spotting

Abstract

A number of caladium cultivars (Caladium ×hortulanum), including Miss Muffet and Gingerland, produce rugose leaves. The rugosity on these leaves is an intriguing characteristic, often resulting in an increased ornamental value. This study was conducted to understand the mode of inheritance of this trait and to determine its genetic relationship with other foliar characteristics including leaf shape, main vein color, and leaf spotting in caladiums. Sixteen caladium cultivars/breeding lines were crossed and 20 populations were produced; progeny of these populations were phenotyped for rugose leaf as well as leaf shape, main vein color, and leaf spotting. Results showed that a single locus with two alleles controlled the presence or absence of rugose leaves in these populations. The locus was designated as RLF, with the dominant RLF allele for rugose leaves and the recessive allele rlf for nonrugose (flat) leaves. Rugose cultivars Miss Muffet and Gingerland and breeding line UF-317 possessed the heterozygous genotype RLFrlf. Rugose leaf was inherited independently from leaf shape, but linked with the green main vein allele (Vg) at the V locus and the leaf spotting allele (S) at the S locus. Three-point analysis of the segregation of the three linked traits in reciprocal crosses between ‘Miss Muffet’ and nonrugose ‘Candidum’ indicated a genetic linkage map with the gene order of S locus locating between the V and the RLF loci. The information obtained from this study will be useful for developing breeding strategies for producing new caladium cultivars with or without rugose leaves, and can facilitate the understanding of the mode of inheritance for rugose leaves in other aroids and other plants.