Inheritance of photoperiod sensitive genic male sterility and breeding of photoperiod sensitive genic male-sterile lines in rice (Oryza sativa L.) through anther culture

Abstract

The fertility segregations of F1, F2, BCF1 descended from crosses between PSGMR and japonica varieties, and F1's anther cultured homozygous diploid pollen plant populations (H2) were studied to reveal the genetic mechanism of photoperiod sensitive genic male sterility in PSGMR under natural daylight length at Shanghai. Rate of bagged seed-setting was used as an indicator of fertility. Fifteen F1 showed complete fertility similar to their parents. The ratio of completely sterile plants to fertile plants in fifteen F2 and four BCF1 was 1:15 and 1:3, respectively. The ratio of completely sterile to fertile diploid pollen plants in nine diploid populations (H2) was 1:3. These results demonstrated that the photoperiod sensitive genic male sterility in PSGMR was governed by two pairs of independent major recessive genes. There were no significant fertility segregations in hybrids F1 and selfed F2 between Nongken 58S and its derivatives 7001S, 5088S, 5047S and M105-9S, indicating that the photoperiod sensitive genic male-sterile genes in Nongken 58S were allelic to those in its derivatives. Several photoperiod sensitive genic male-sterile diploid pollen lines were bred from anther cultured homozygous diploid populations (H2) in about a three-year period. Most of these diploid lines showed significant fertility transformation and stable complete sterility from 5 August to 5 September, excellent agronomic traits and high resistance to blast and bacterial leaf blight.