Inheritance of downy mildew resistance at different developmental stages in Chinese cabbage via the leaf disk test

Abstract

Downy mildew, caused by Hyaloperonospora parasitica, is a destructive disease of the Chinese cabbage (Brassica rapa ssp. pekinensis). A rapid resistance/susceptibility test for H. parasitica was established by the inoculation of a leaf disk test. Four conditions were tested and the optimal condition was found when the inoculated leaf disk was placed into dark conditions at 16°C for the first 24 hours after inoculation, followed by 5 days of light at 20 and 23°C for 4 hours and 5 hours, respectively, dark at 16°C and 12°C for 3 hours and 12 hours, respectively, and a final temperature of 16°C for 24 hours. There was a good correlation between the resistance levels of leaf disks and the resistance of seedlings or adult plants, which indicated that testing leaf disks should be the preferred methods to predict the resistance of adult plants. Using this method, downy mildew resistance was investigated in a double haploid (DH) population at four developmental stages. The results showed that the genetic pattern, which was deduced from the DH segregation, was relatively similar, but varied slightly during plant development.