Abstract
The objective of this study was to determine whether it was possible to develop in soybean [G. max (L.) Merr.] seeds double null genotypes for the Kunitz trypsin inhibitor (ti) null in reciprocal combinations with genotypes lacking lectin (le), lipoxygenase‐1 (/xl), μ‐amylase (sp1), and urease (Eul‐sun). Homozygous double recessive soybean lines were obtained for all eight combinations. From F2 data collected using colorimetric, immunological, and electrophoretic tests, we demonstrated that the Kunitz trypsin inhibitor locus segregated independently of the lectin, lipoxygenase‐1, urease and α‐amylase loci.
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