Abstract
The inheritance of 27 loci from 18 enzyme systems was investigated using both megagametophyte and embryo tissues of open pollinated seed collected from a natural stand of coastal Douglas-fir [Pseudotsuga menziesii var. menziesii (Mirb.) Franco]. Four enzyme systems - glutamate dehydrogenase (GDH), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), mannose-6-phosphate isomerase (MPI), and peptidase (PEP) - appeared to be monomorphic and the remaining 14 systems - acid phosphatase (APH), aconitase (ACO), aspartate aminotransferase (AAT), diaphorase (DIA), esterase (EST), glucose-6-phosphate dehydrogenase (G6P), hexoseaminidase (HA), isocitrate dehydrogenase (IDH), malate dehydrogenase (MDH), malic enzyme (ME), phosphoglucose isomerase (PGI), phosphoglucomutase (PGM), 6-phosphogluconic dehydrogenase (6-PGD), and superoxide dismutase (SOD)-showed polymorphism. Each enzyme system was discussed with respect to its multilocus organization, subunit (quaternary) structure, and allelic variation.
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